Disposition kinetics of long acting moxifloxacin following intravenous administration in Sheep

Aim: The objective of the present study was to study the disposition kinetics and dosage regimens of long acting moxifloxacin -1 following intravenous administration at the dose rate of 7.5 mg/kg b. wt. in six male sheep and to calculate dosage regimens of the same in sheep. Materials and Methods: The study was conducted using six healthy male sheep. Long acting Moxifloxacin solution (10 % moxifloxacin in solution with Larginine, N-butyl alcohol and benzyl alcohol) was injected in jugular vein and periodical blood samples were collected from contra-lateral jugular vein in test tubes containing 30-50 IU heparin (anticoagulant) at 0.083 (5 min), 0.166 (10 min), 0.5, 1, 2, 4, 8, 12, 24, 36, 48, 60, 72 and up to 96 h post administration of drug. Drug concentration in plasma was determined using High Performance Liquid Chromatography (HPLC) with Fluorescence Detector. The blood concentrations versus time data were analyzed using software. Results: After single dose intravenous administration of long acting moxifloxacin the plasma concentration of 0.016 ± 0.001 -1 μg/ml was maintained for up to 72 h. Distribution half-life (t ) and elimination half-life (t ) were 1.637 ± 0.053 h, and 1/2 1/2 -1 12.130 ± 0.202 h, following IV administration. The mean values of apparent volume of distribution V 5.436 ± 0.135 L/kg d(area) as well as mean residence time 10.02 ± 4.787 minute were detected with IV administration. Conclusion: The long acting Moxifloxacin @ the dose 7.5 mg/kg IV maintains the effective therapeutic concentration in the plasma of sheep for up to 72 hours. The long acting Moxifloxacin at this dose rate can be used to treat sensitive bacteria causing infectious diseases in sheep.